Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China     

Jun Wang  Qingjuan Tang  Zhiqin Yue  Zhaojie Li  Jin Zhang  Changhu Xue 《中国海洋大学学报(英文版)》2008,7(2):223-227

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.  相似文献   

鲤鱼（Cyprinuscarpio L．）体重和体长QTL的定位   总被引：1，自引：0，他引：1  

刘继红  ;张研  ;常玉梅  ;梁利群  ;张晓峰  ;鲁翠云  ;徐美佳  ;孙效文 《广东海洋大学学报》2009,(4):19-25

利用265个AFLP标记、127个微卫星分子标记、37个EST-SSR标记和16个RAPD标记（共445个标记）对大头鲤／荷包红鲤抗寒品系的F2代雌核发育群体44个个体进行基因型检测，构建鲤鱼（CyprinuscarpioL.）遗传连锁图谱；利用软件WinQTLCart2.5采用复合区间作图法对体重和体长两个性状进行了QTL定位分析。结果共检测到与两个与体重性状相关的QTL，分别定位到LG24连锁群（qBWh-24-1）和LG20连锁群（qBWh-20-1）上，可解释的表型变异分别为7.98％和20.05％；检测到两个与体长性状相关的QTL，分别位于LG2连锁群（qFS-2-1）和LG20连锁群（qFS-20-1），可解释的表型变异分别为5.69％和12.69％，4个QTL的加性效应均为负值。  相似文献   

Synthetic information prediction system for crisis mine based on GIS     

Yuxin Ye  Ping Yu  Shi Wang  Shuisheng Ye 《世界地质(英文版)》2006,9(1)

Reserves of some kinds of the crisis mines will be lack now or from now on, because of lacking seriously reserves of mineral resources and the crisis of exploring bases in support. So that it is urgent to predict, appraise, development and utilize the replaceable resources of the crisis mines. The mineral resources prediction software system of synthetic information is intelligent GIS which is used to quantitative prediction of large-scale synthetic information mineral target. It takes the geological body and the mineral resource body as a unit. And it analyzes the ore deposit genesis and metallotect, knows the spatial distribution laws of the ore deposit and ore body, and establish the prospecting model based on the concept of establishing the three-dimensional space of a mine. This paper will primarily discuss some important problems as follows: the secondary development of various kinds of data(including geology, geophysical prospecting, geochemical prospecting and remote sensing, etc); process synthetically and establish the synthetic information interpretative map base; correspond prospecting model with synthetic information of ore deposit; divided into statistical units of metallogenic information synthetic anomalies based on the synthetic information anomalies of ore control, then research the metallogenic information variable of unit synthetically and make quantitative prediction according to choose the quantitative prediction math model which is suitable to the demands of large-scale precision; at last, finish the target area optimization of ore deposit (body).  相似文献   

Fast tidal cycling and the origin of life     

Richard Lathe 《Icarus》2004,168(1):18-22

Replicating prebiotic polymers are thought to predate the emergence of true life-forms. The initial mode of replication, a prerequisite for Darwinian selection, is unknown, but demands an explanation based on local physicochemistry. Dual consideration of the conditions of the early terrestrial surface, with the unusual physicochemical properties of nucleic acids like DNA, could explain the emergence of nucleic acids as key biomolecules. The early impact that produced the Moon, and fast terrestrial rotation, subjected coastal areas 3.9 Ga ago to rapid tidal flooding (dilution) and drying (concentration), with a likely periodicity in the range of 2-6 h, and could have provided a driving force for cyclic replication of early biomolecules. Such a mechanism applies only to molecules capable of association/polymerization at high salt concentration, and of dissociation at low salinity. Nucleic acids meet these criteria. It is suggested that tidal cycling, resembling the polymerase chain reaction (PCR) mechanism, could only replicate and amplify DNA-like polymers. This mechanism suggests constraints on the evolution of extra-terrestrial life.  相似文献   

斑节对虾白斑综合症杆状病毒感染的实验模式     

邱德全  林继辉  刘丽  张凌飞 《广东海洋大学学报》2001,21(1):10-13

在建立斑节对虾实验室养殖模式的基础上，对野外采集的有白斑综合症病毒（WSSV）病典型症状的斑节对虾初步纯化其病毒，并进行WSSV的PCR检测，得到WSSV的感染用样品。对健康斑节对虾分别进行浸浴感染，投喂感染和注射感染。对感染死亡个体进行WSSA的PCR检测和细菌检测，证实WSSV感染性和致死性。浸浴感染、投喂感染和注射感染的感染量分别为4mL/L、0.2g/10g虾体、1/2稀释液0.05mL/10g虾体，死亡开始时间分别为16d、42h、28h，三种感染模式最终死亡率100%，从开始死亡到全部死亡延续时间分别为15d、82h、44h。  相似文献   

实验条件对X射线衍射物相定量分析结果的影响   总被引：8，自引：6，他引：2       下载免费PDF全文

房俊卓  张霞  徐崇福 《岩矿测试》2008,27(1):60-62

通过实验验证了多物相试样X射线衍射定量分析的基体效应,着重研究了参比强度K值、衍射强度、混合物各物相粒度、X射线管功率等实验条件对物相定量分析结果的影响。结果表明,物相定性分析是定量分析的前提,采用积分强度、使各物相颗粒尽可能细而且均匀、采用较高的X射线管功率以及适合的扫描速率均有利于提高物相X射线衍射定量分析的准确度。  相似文献   

土体微观结构定量分析系统及应用   总被引：8，自引：0，他引：8  

张礼中  胡瑞林  李向全  张永波 《地质科技情报》2008,27(1):108-112

土体微观结构图像的计算机处理是土体微观结构定量化研究的关键技术之一.在分析我国土体微观结构定量研究的进展的基础上,针对土体微观结构图像计算机处理软件对土体微观结构非确定型参数定量分析功能的不足,设计和开发了新版土体微观结构定量分析系统,以太原高阶地黄土为例,通过各夯击次数对应土样的定量化结构效应分析,探讨了其动力固结的微观结构变化规律.该系统的应用对推动我国土体微观结构的研究具有积极意义.  相似文献   

河南省森林火险月季尺度定量客观化预测          下载免费PDF全文

吴璐 《气象与环境科学》2018,41(2):93-99

基于火灾资料和气象资料,利用数理统计方法和百分位法,分析了河南省林区火灾的气候特征及防火关键期高火险日数的月、季气候特征。结果表明,林区火灾次数年际波动较大,主要出现在每年的冬春季(12月次年4月),3月出现的次数最多,2月的次之。火灾峰值主要出现在13-16时。3月高火险日数存在显著增加的线性趋势,12月和1月高火险日数呈现先减少后增加的趋势。2月和4月高火险日数处在偏少的年代际背景下。林区春季高火险日数存在显著增加的线性趋势,在桐柏-大别山林区尤其明显。选取河南省防火关键期各月、季高火险日数与海温、积雪、环流场等130项环流指数的相关关系最好的因子,利用最优子集回归分别建立基于林区内各个气象站点的预测方程。通过回代检验和趋势异常综合评分(Ps),对回归方程预测效果进行检验,结果显示,回代曲线和历史曲线吻合好,表明预测方程预测结果接近实况。  相似文献   

试论地貌学的新进展和趋势   总被引：1，自引：0，他引：1  

鹿化煜 《地理科学进展》2018,37(1):8-15

地貌变化有内在的规律,对环境保育、资源利用和自然灾害防治有重要影响。作为交叉学科和地理学的重要分支学科,地貌学具有重要理论价值和实际意义。过去10多年来,随着遥感、地理信息技术和沉积物定年、地球物理和地球化学探测以及数值模拟等新技术和新方法的应用,地貌学家的研究视野和深度不断扩大与加深,在构造地貌、气候地貌和人类活动与地貌过程等传统领域有很多新进展,揭示了不同地貌单元的变化规律和机理,极大地推动了地貌学的发展。在新时代,除了继续加强传统地貌单元的深入研究外,地貌学的发展应与全球环境变化和未来地球等重大科学问题(计划)紧密结合;地貌学家要关心人类活动对地貌过程的影响及适应,并利用新技术开展地貌过程的定量重建和数值模拟。同时加强地理学专业学生的地质学基础学习、发展定量地貌学和行星地貌学、系统开展人类活动对地貌过程的影响和适应研究,是提升中国地貌学水平的途径,也可为推动国际地貌学发展做出贡献。  相似文献   

Quantitative analysis of the impact factors of conventional energy carbon emissions in Kazakhstan based on LMDI decomposition and STIRPAT model   总被引：2，自引：2，他引：0  

Jiaxiu Li  Yaning Chen  Zhi Li  Zhihui Liu 《地理学报(英文版)》2018,28(7):1001-1019

Quantitative analysis of the impact factors in energy-related CO₂ emissions serves as an important guide for reducing carbon emissions and building an environmentally-friendly society. This paper aims to use LMDI method and a modified STIRPAT model to research the conventional energy-related CO₂ emissions in Kazakhstan after the collapse of the Soviet Union. The results show that the trajectory of CO₂ emissions displayed U-shaped curve from 1992 to 2013. Based on the extended Kaya identity and additive LMDI method, we decomposed total CO₂ emissions into four influencing factors. Of those, the economic active effect is the most influential factor driving CO₂ emissions, which produced 110.86 Mt CO₂ emissions, with a contribution rate of 43.92%. The second driving factor is the population effect, which led to 11.87 Mt CO₂ emissions with a contribution rate of 4.7%. On the contrary, the energy intensity effect is the most inhibiting factor, which caused–110.90 Mt CO₂ emissions with a contribution rate of–43.94%, followed by the energy carbon structure effect resulting in–18.76 Mt CO₂ emissions with a contribution rate of–7.43%. In order to provide an in-depth examination of the change response between energy-related CO₂ emissions and each impact factor, we construct a modified STIRPAT model based on ridge regression estimation. The results indicate that for every 1% increase in population size, economic activity, energy intensity and energy carbon structure, there is a subsequent increase in CO₂ emissions of 3.13%, 0.41%, 0.30% and 0.63%, respectively.  相似文献